HMGCR Kit ELISA

N° du produit ABIN5674904

Détail du produit HMGCR Kit ELISA

Antigène: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.31 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Autre désignation: 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) (HMGCR Produits)

Synonymes: HMGR Kit ELISA, LDLCQ3 Kit ELISA, hmg-coa-r Kit ELISA, HMG-CoAR Kit ELISA, Red Kit ELISA, 3H3M Kit ELISA, BcDNA:LD15354 Kit ELISA, CG10367 Kit ELISA, Clb Kit ELISA, DmHMG-CoAr Kit ELISA, Dmel\\CG10367 Kit ELISA, HMGCR Kit ELISA, HMGCR_Dm Kit ELISA, HMGCoAR Kit ELISA, HMGCoARr Kit ELISA, HMGCoAr Kit ELISA, HmG-CoAR Kit ELISA, Hmg Kit ELISA, bro1 Kit ELISA, bs32f05.y1 Kit ELISA, clb Kit ELISA, hmgc Kit ELISA, hmgcr Kit ELISA, hmgrc Kit ELISA, l(3)01152 Kit ELISA, l(3)04684 Kit ELISA, hmgcr2 Kit ELISA, im:6912716 Kit ELISA, zgc:110746 Kit ELISA, 3-hydroxy-3-methylglutaryl-CoA reductase Kit ELISA, 3-hydroxy-3-methylglutaryl-CoA reductase L homeolog Kit ELISA, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Kit ELISA, HMG Coenzyme A reductase Kit ELISA, 3-hydroxy-3-methylglutaryl-CoA reductase b Kit ELISA, HMGCR Kit ELISA, hmgcr.L Kit ELISA, Hmgcr Kit ELISA, hmgcrb Kit ELISA

Sujet: Alternative name: HMG-CoA Reductase, Hydroxymethylglutaryl-CoA Reductase

ID gène: 15357

UniProt: Q01237

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha