PAX6 Kit ELISA

N° du produit ABIN5674917

Détail du produit PAX6 Kit ELISA

Antigène: PAX6 (Paired Box 6 (PAX6))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0124 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PAX6 (Paired Box 6 (PAX6))

Autre désignation: Paired Box Gene 6 (PAX6) (PAX6 Produits)

Synonymes: pax6 Kit ELISA, an2 Kit ELISA, mgda Kit ELISA, wagr Kit ELISA, pax-6 Kit ELISA, pax6a Kit ELISA, xpax6 Kit ELISA, XLPAX6 Kit ELISA, MGC52531 Kit ELISA, PAX-6 Kit ELISA, AN Kit ELISA, AN2 Kit ELISA, D11S812E Kit ELISA, MGDA Kit ELISA, WAGR Kit ELISA, Pax-6 Kit ELISA, pax6b Kit ELISA, 1500038E17Rik Kit ELISA, AEY11 Kit ELISA, Dey Kit ELISA, Gsfaey11 Kit ELISA, Sey Kit ELISA, PAXZF-A Kit ELISA, Pax6.1 Kit ELISA, cb280 Kit ELISA, fc20e07 Kit ELISA, pax-a Kit ELISA, pax[zf-a] Kit ELISA, paxzfa Kit ELISA, wu:fc20e07 Kit ELISA, zfpax-6a Kit ELISA, zfpax-6b Kit ELISA, paired box 6 Kit ELISA, paired box 6 L homeolog Kit ELISA, paired box 6 S homeolog Kit ELISA, paired box 6a Kit ELISA, PAX6 Kit ELISA, pax6 Kit ELISA, Pax6 Kit ELISA, pax6.L Kit ELISA, pax6.S Kit ELISA, pax6a Kit ELISA

Sujet: Alternative name: AN, AN2, MGDA, WAGR, Oculorhombin, Aniridia,Keratitis, Aniridia type II protein

ID gène: 18508

UniProt: P63015

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis