PDE3B Kit ELISA

N° du produit ABIN5674920

Détail du produit PDE3B Kit ELISA

Antigène: PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 26.35 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))

Autre désignation: Phosphodiesterase 3B, cGMP Inhibited (PDE3B) (PDE3B Produits)

Synonymes: PDE3B Kit ELISA, HcGIP1 Kit ELISA, cGIPDE1 Kit ELISA, 9830102A01Rik Kit ELISA, AI847709 Kit ELISA, phosphodiesterase 3B Kit ELISA, phosphodiesterase 3B, cGMP-inhibited Kit ELISA, si:ch211-79e4.4 Kit ELISA, phosphodiesterase 3B L homeolog Kit ELISA, PDE3B Kit ELISA, pde3b Kit ELISA, Pde3b Kit ELISA, si:ch211-79e4.4 Kit ELISA, pde3b.L Kit ELISA

Sujet: Alternative name: HcGIP1, cGIPDE1, CGIPDE1, CGIP1, CGI-PDE B, Cyclic GMP-inhibited phosphodiesterase B

ID gène: 5140

UniProt: Q13370

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process