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SPTLC1 Kit ELISA

SPTLC1 Reactivité: Humain Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Tissue Homogenate
N° du produit ABIN5674929
  • Antigène Voir toutes SPTLC1 Kits ELISA
    SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78.12 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78.12 pg/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    27 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes SPTLC1 Kits ELISA
    SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
    Abstract
    SPTLC1 Produits
    Synonymes
    ATSPT1 Kit ELISA, SERINE PALMITOYLTRANSFERASE 1 Kit ELISA, serine palmitoyltransferase 1 Kit ELISA, Afu6g00300 Kit ELISA, HSAN1 Kit ELISA, HSN1 Kit ELISA, LBC1 Kit ELISA, LCB1 Kit ELISA, SPT1 Kit ELISA, SPTI Kit ELISA, wu:fc75h04 Kit ELISA, wu:fp41c08 Kit ELISA, zgc:112247 Kit ELISA, AW552086 Kit ELISA, C77762 Kit ELISA, E030036H05 Kit ELISA, Lcb1 Kit ELISA, RGD1306617 Kit ELISA, Spt1 Kit ELISA, serine palmitoyltransferase 1 Kit ELISA, Serine palmitoyltransferase 1 Kit ELISA, serine palmitoyltransferase long chain base subunit 1 Kit ELISA, serine palmitoyltransferase, long chain base subunit 1 Kit ELISA, serine palmitoyltransferase, long chain base subunit 1 L homeolog Kit ELISA, SPT1 Kit ELISA, AFUA_6G00300 Kit ELISA, PAS_FragB_0040 Kit ELISA, SPTLC1 Kit ELISA, sptlc1 Kit ELISA, sptlc1.L Kit ELISA, Sptlc1 Kit ELISA
    Sujet
    Alternative name: HSAN, HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI, Hereditary Sensory Neuropathy,Type 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1
    ID gène
    10558
    UniProt
    O15269
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