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GATA1 Kit ELISA

GATA1 Reactivité: Humain Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
N° du produit ABIN5690754
  • Antigène Voir toutes GATA1 Kits ELISA
    GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
    Reactivité
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    DNA-Binding ELISA
    Application
    ELISA
    Fonction
     Human GATA-1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA1 binding sequences to semi-quantitatively detect active GATA1 in lysates or nuclear extracts.
    Type d'échantillon
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificité
    The olionucleotide/antibody pair provided in this kit recognizes human GATA-1 in whole lysates and nuclear extracts.
    Attributs du produit
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Ingrédients
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes GATA1 Kits ELISA
    GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
    Autre désignation
    GATA1 (GATA1 Produits)
    Synonymes
    ERYF1 Kit ELISA, GATA-1 Kit ELISA, GF-1 Kit ELISA, GF1 Kit ELISA, NF-E1 Kit ELISA, NFE1 Kit ELISA, XLANP Kit ELISA, XLTDA Kit ELISA, XLTT Kit ELISA, Gata-1 Kit ELISA, Gf-1 Kit ELISA, eryf1 Kit ELISA, Eryf1 Kit ELISA, GATA1 Kit ELISA, gata-1 Kit ELISA, Gata1 Kit ELISA, si:dkeyp-77f7.5 Kit ELISA, gata1 Kit ELISA, gata1a-A Kit ELISA, xGATA-1 Kit ELISA, xgata1 Kit ELISA, XGATA-1B Kit ELISA, gata1b-A Kit ELISA, gta1 Kit ELISA, zg1 Kit ELISA, NF-E1a Kit ELISA, GATA binding protein 1 Kit ELISA, GATA binding protein 1 (globin transcription factor 1) Kit ELISA, amidase Kit ELISA, aspartyl/glutamyl-tRNA amidotransferase subunit A Kit ELISA, putative amidase Kit ELISA, GatA1 Kit ELISA, glutamyl-tRNA amidotransferase subunit A Kit ELISA, hematopietic transcription factor GATA-1 Kit ELISA, GATA binding protein 1b Kit ELISA, GATA binding protein 1 L homeolog Kit ELISA, GATA binding protein 1 S homeolog Kit ELISA, GATA binding protein 1a Kit ELISA, erythroid transcription factor Kit ELISA, GATA1 Kit ELISA, Gata1 Kit ELISA, gatA1 Kit ELISA, gata-1 Kit ELISA, gata1b Kit ELISA, gata1.L Kit ELISA, gata1.S Kit ELISA, gata1a Kit ELISA, LOC108348091 Kit ELISA
    ID gène
    2623
    UniProt
    P15976
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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