ACP6 Kit ELISA

N° du produit ABIN5690814

Détail du produit ACP6 Kit ELISA

Antigène: ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ACP6 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human ACP6.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))

Autre désignation: ACP6 (ACP6 Produits)

Synonymes: ACP6 Kit ELISA, im:7147584 Kit ELISA, zgc:172268 Kit ELISA, MGC146066 Kit ELISA, ACPL1 Kit ELISA, LPAP Kit ELISA, PACPL1 Kit ELISA, 5730559A09Rik Kit ELISA, AU022842 Kit ELISA, mPACPL1 Kit ELISA, acid phosphatase 6, lysophosphatidic Kit ELISA, acid phosphatase 6, lysophosphatidic S homeolog Kit ELISA, ACP6 Kit ELISA, acp6 Kit ELISA, acp6.S Kit ELISA, Acp6 Kit ELISA

ID gène: 51205

UniProt: Q9NPH0