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C9orf3 Kit ELISA

C9orf3 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690820
  • Antigène Voir toutes C9orf3 Kits ELISA
    C9orf3 (Chromosome 9 Open Reading Frame 3 (C9orf3))
    Reactivité
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Aminopeptidase O ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Aminopeptidase O.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes C9orf3 Kits ELISA
    C9orf3 (Chromosome 9 Open Reading Frame 3 (C9orf3))
    Autre désignation
    Aminopeptidase O (C9orf3 Produits)
    Synonymes
    apo Kit ELISA, ap-o Kit ELISA, aopep Kit ELISA, MGC68599 Kit ELISA, Aopep Kit ELISA, Apo Kit ELISA, RGD1309592 Kit ELISA, AOPEP Kit ELISA, AP-O Kit ELISA, APO Kit ELISA, C90RF3 Kit ELISA, ONPEP Kit ELISA, 2300006M17Rik Kit ELISA, chromosome 9 open reading frame 3 L homeolog Kit ELISA, chromosome 9 open reading frame 3 Kit ELISA, aminopeptidase O Kit ELISA, RIKEN cDNA 2010111I01 gene Kit ELISA, c9orf3.L Kit ELISA, c9orf3 Kit ELISA, Npepo Kit ELISA, C9orf3 Kit ELISA, 2010111I01Rik Kit ELISA
    ID gène
    84909
    UniProt
    Q8N6M6
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