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APLP1 Kit ELISA

APLP1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690822
  • Antigène Voir toutes APLP1 Kits ELISA
    APLP1 (Amyloid beta (A4) Precursor-Like Protein 1 (APLP1))
    Reactivité
    • 2
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Amyloid-like protein 1/APLP-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Amyloid-like protein 1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes APLP1 Kits ELISA
    APLP1 (Amyloid beta (A4) Precursor-Like Protein 1 (APLP1))
    Autre désignation
    Amyloid-like protein 1 (APLP-1 (APLP1 Produits)
    Synonymes
    aplp Kit ELISA, MGC115336 Kit ELISA, APLP Kit ELISA, Aplp1_retired Kit ELISA, RGD1561211 Kit ELISA, amyloid beta precursor like protein 1 Kit ELISA, amyloid beta precursor like protein 1 L homeolog Kit ELISA, amyloid beta (A4) precursor-like protein 1 Kit ELISA, APLP1 Kit ELISA, aplp1.L Kit ELISA, aplp1 Kit ELISA, Aplp1 Kit ELISA
    ID gène
    333
    UniProt
    P51693
    Pathways
    cAMP Metabolic Process
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