Catalase Kit ELISA

N° du produit ABIN5690839

Détail du produit Catalase Kit ELISA

Antigène: Catalase (CAT)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Catalase ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Catalase.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Catalase (CAT)

Autre désignation: Catalase (CAT Produits)

Synonymes: 2210418N07 Kit ELISA, Cas-1 Kit ELISA, Cas1 Kit ELISA, Cs-1 Kit ELISA, CS1 Kit ELISA, Cat01 Kit ELISA, Catl Kit ELISA, fb68a12 Kit ELISA, wu:fb68a12 Kit ELISA, CAT Kit ELISA, CATA Kit ELISA, CG6871 Kit ELISA, CT21282 Kit ELISA, CatA Kit ELISA, DMCATHPO Kit ELISA, DROCATHPO Kit ELISA, Dmel\\CG6871 Kit ELISA, U00145 Kit ELISA, bs36h11.y1 Kit ELISA, cat Kit ELISA, GB11648 Kit ELISA, catalase Kit ELISA, Cat Kit ELISA, BA0843 Kit ELISA, DKFZp469E0232 Kit ELISA, catalase Kit ELISA, Catalase Kit ELISA, catalase, gene 2 Kit ELISA, CAT Kit ELISA, Cat Kit ELISA, cat Kit ELISA, cat.2 Kit ELISA, LOC769804 Kit ELISA, BA_0843 Kit ELISA

ID gène: 847

UniProt: P04040

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism