CDK5R1 Kit ELISA

N° du produit ABIN5690848

Détail du produit CDK5R1 Kit ELISA

Antigène: CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human CDK5 Activator 1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human CDK5 Activator 1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))

Autre désignation: CDK5 Activator 1 (CDK5R1 Produits)

Synonymes: CDK5P35 Kit ELISA, CDK5R Kit ELISA, NCK5A Kit ELISA, p23 Kit ELISA, p25 Kit ELISA, p35 Kit ELISA, p35nck5a Kit ELISA, cdk5r1 Kit ELISA, zgc:92814 Kit ELISA, CDK5R1 Kit ELISA, Cdk5r Kit ELISA, P25/P35 Kit ELISA, D11Bwg0379e Kit ELISA, Xp35.1 Kit ELISA, cyclin dependent kinase 5 regulatory subunit 1 Kit ELISA, cyclin-dependent kinase 5, regulatory subunit 1b (p35) Kit ELISA, cyclin-dependent kinase 5 regulatory subunit 1 Kit ELISA, S100 calcium binding protein A9 Kit ELISA, cyclin-dependent kinase 5, regulatory subunit 1 (p35) Kit ELISA, cyclin-dependent kinase 5, regulatory subunit 1 (p35) L homeolog Kit ELISA, CDK5R1 Kit ELISA, cdk5r1b Kit ELISA, Cdk5r1 Kit ELISA, S100A9 Kit ELISA, cdk5r1.L Kit ELISA

Classe de substances: Viral Protein

ID gène: 8851

UniProt: Q15078

Pathways: Stem Cell Maintenance, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity