Choline Acetyltransferase Kit ELISA
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- Antigène Voir toutes Choline Acetyltransferase (CHAT) Kits ELISA
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human ChAT/Choline acetylase/CHOACTase ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human ChAT.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 6 months
-
- Antigène Voir toutes Choline Acetyltransferase (CHAT) Kits ELISA
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
- Autre désignation
- ChAT (Choline acetylase/CHOACTase) (CHAT Produits)
- Synonymes
- CHOACTASE Kit ELISA, CMS1A Kit ELISA, CMS1A2 Kit ELISA, ChoAcTase Kit ELISA, B230380D24Rik Kit ELISA, choline O-acetyltransferase Kit ELISA, choline O-acetyltransferase a Kit ELISA, choline acetyltransferase Kit ELISA, carnitine O-acetyltransferase Kit ELISA, CHAT Kit ELISA, chata Kit ELISA, Chat Kit ELISA, PGTG_00551 Kit ELISA, LOC100533322 Kit ELISA
- ID gène
- 1103
- UniProt
- P28329
- Pathways
- Skeletal Muscle Fiber Development
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