DIDO1 Kit ELISA

N° du produit ABIN5690865

Détail du produit DIDO1 Kit ELISA

Antigène: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human DIDO1/DIO-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human DIDO1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Autre désignation: DIDO1 (DIO-1 (DIDO1 Produits)

Synonymes: 5DI Kit ELISA, TXDI1 Kit ELISA, BYE1 Kit ELISA, C20orf158 Kit ELISA, DATF-1 Kit ELISA, DATF1 Kit ELISA, DIDO2 Kit ELISA, DIDO3 Kit ELISA, DIO-1 Kit ELISA, DIO1 Kit ELISA, dJ885L7.8 Kit ELISA, 6720461J16Rik Kit ELISA, C530043I07 Kit ELISA, D130048F08Rik Kit ELISA, Datf1 Kit ELISA, Dido2 Kit ELISA, Dido3 Kit ELISA, mKIAA0333 Kit ELISA, DIDO1 Kit ELISA, datf1l Kit ELISA, fi35b04 Kit ELISA, wu:fi35b04 Kit ELISA, zgc:158157 Kit ELISA, DKFZp469J1523 Kit ELISA, bye1 Kit ELISA, dio1 Kit ELISA, datf1 Kit ELISA, dido2 Kit ELISA, dido3 Kit ELISA, dio-1 Kit ELISA, iodothyronine deiodinase 1 Kit ELISA, death inducer-obliterator 1 Kit ELISA, death inducer-obliterator 1 L homeolog Kit ELISA, DIO1 Kit ELISA, DIDO1 Kit ELISA, Dido1 Kit ELISA, dido1.L Kit ELISA, dido1 Kit ELISA

ID gène: 11083

UniProt: Q9BTC0

Pathways: Hormone Activity