GLB1 Kit ELISA

N° du produit ABIN5690881

Détail du produit GLB1 Kit ELISA

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GLB1/Beta-galactosidase ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human GLB1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: GLB1 (Beta-galactosidase (GLB1 Produits)

Synonymes: EBP Kit ELISA, ELNR1 Kit ELISA, MPS4B Kit ELISA, DER Kit ELISA, XR Kit ELISA, 0610038K04Rik Kit ELISA, 1810027P18Rik Kit ELISA, glb Kit ELISA, CG9092 Kit ELISA, Dmel\\CG9092 Kit ELISA, beta-GAL Kit ELISA, beta-Gal-1 Kit ELISA, beta-gal Kit ELISA, gal Kit ELISA, lacZ-1 Kit ELISA, AW125515 Kit ELISA, Bge Kit ELISA, Bgl Kit ELISA, Bgl-e Kit ELISA, Bgl-s Kit ELISA, Bgl-t Kit ELISA, Bgs Kit ELISA, Bgt Kit ELISA, C130097A14Rik Kit ELISA, BGAL Kit ELISA, zgc:110823 Kit ELISA, ECK0341 Kit ELISA, JW0335 Kit ELISA, GLB Kit ELISA, galactosidase beta 1 Kit ELISA, dicarbonyl and L-xylulose reductase Kit ELISA, dicarbonyl L-xylulose reductase Kit ELISA, beta-D-galactosidase Kit ELISA, beta-galactosidase Kit ELISA, beta galactosidase Kit ELISA, galactosidase, beta 1 Kit ELISA, beta-galactosidase-like Kit ELISA, GLB1 Kit ELISA, DCXR Kit ELISA, Dcxr Kit ELISA, lacZ Kit ELISA, SSP_RS00525 Kit ELISA, lacH Kit ELISA, ECL_03691 Kit ELISA, Gal Kit ELISA, Glb1 Kit ELISA, glb1 Kit ELISA, VV2_1327 Kit ELISA, LOC100056371 Kit ELISA

ID gène: 2720

UniProt: P16278

Pathways: Glycosaminoglycan Metabolic Process