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SUMF2 Kit ELISA

SUMF2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690957
  • Antigène Voir toutes SUMF2 Kits ELISA
    SUMF2 (Sulfatase Modifying Factor 2 (SUMF2))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human SUMF2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human SUMF2.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes SUMF2 Kits ELISA
    SUMF2 (Sulfatase Modifying Factor 2 (SUMF2))
    Autre désignation
    SUMF2 (SUMF2 Produits)
    Synonymes
    MGC82506 Kit ELISA, zgc:103574 Kit ELISA, SUMF2 Kit ELISA, sumf2 Kit ELISA, DKFZp468M2113 Kit ELISA, pFGE Kit ELISA, 2610040F05Rik Kit ELISA, sulfatase modifying factor 2 Kit ELISA, sulfatase modifying factor 2 L homeolog Kit ELISA, SUMF2 Kit ELISA, sumf2.L Kit ELISA, sumf2 Kit ELISA, Sumf2 Kit ELISA
    ID gène
    25870
    UniProt
    Q8NBJ7
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