Tryptophan Hydroxylase 1 Kit ELISA

N° du produit ABIN5690961

Détail du produit Tryptophan Hydroxylase 1 Kit ELISA

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human TPH-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human TPH-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Autre désignation: TPH-1 (TPH1 Produits)

Synonymes: TPRH Kit ELISA, TRPH Kit ELISA, tph Kit ELISA, tph1 Kit ELISA, tphD1 Kit ELISA, tph2 Kit ELISA, tph1l Kit ELISA, tphD2 Kit ELISA, Tph Kit ELISA, TPH Kit ELISA, SERGEF Kit ELISA, LOC100229598 Kit ELISA, tryptophan hydroxylase 1 Kit ELISA, tryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a Kit ELISA, TryPtophan Hydroxylase Kit ELISA, tryptophan hydroxylase 1b Kit ELISA, tryptophan hydroxylase 1 L homeolog Kit ELISA, tryptophan 5-hydroxylase 1 Kit ELISA, TPH1 Kit ELISA, tph1a Kit ELISA, tph-1 Kit ELISA, tph1b Kit ELISA, Tph1 Kit ELISA, tph1.L Kit ELISA, LOAG_02149 Kit ELISA, Tsp_05203 Kit ELISA

ID gène: 7166

UniProt: P17752

Pathways: Feeding Behaviour