HYAL1 Kit ELISA

N° du produit ABIN5692203

Kit ELISA Humain HYAL1, test Colorimetric pour la quantification de Humain HYAL1.

Aperçu rapide pour HYAL1 Kit ELISA (ABIN5692203)

Antigène: HYAL1 (Hyaluronidase-1 (HYAL1))

Épitope: AA 22-435

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA - heparin), Serum

Détail du produit HYAL1 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Hyaluronidase1/HYAL1. 96wells/kit, with removable strips.

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO, Immunogen sequence: F22-W435

Sensibilité: < 10 pg/mL

Ingrédients: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Information d'application

Durée du test: 15 min

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Date de péremption: 12 months

Détail du antigène

Antigène: HYAL1 (Hyaluronidase-1 (HYAL1))

Autre désignation: HYAL1

Sujet:

Synonyms: Hyaluronidase-1, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1, HYAL1, LUCA1

Tissue Specificity: Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.

Background: Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic  pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.

Cellular Localisation: Secreted

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process