CDCP1 Kit ELISA

N° du produit ABIN5692215

Détail du produit CDCP1 Kit ELISA

Antigène: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Épitope: AA 30-667

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CDCP1. 96wells/kit, with removable strips.

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO, Immunogen sequence: F30-T667

Sensibilité: < 2 pg/mL

Ingrédients: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Information d'application

Durée du test: 15 min

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Date de péremption: 12 months

Détail du antigène

Antigène: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Autre désignation: CDCP1 (CDCP1 Produits)

Synonymes: CD318 Kit ELISA, SIMA135 Kit ELISA, TRASK Kit ELISA, 9030022E12Rik Kit ELISA, AA409659 Kit ELISA, E030027H19Rik Kit ELISA, RGD1305578 Kit ELISA, zgc:113071 Kit ELISA, CUB domain containing protein 1 Kit ELISA, CUB domain containing protein 1a Kit ELISA, CDCP1 Kit ELISA, Cdcp1 Kit ELISA, cdcp1a Kit ELISA

Sujet:

Synonyms: CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with src kinases, CD318, CDCP1, TRASK, UNQ2486/PRO5773

Tissue Specificity: Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.

Background: CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

Cellular Localisation: Isoform 1: Cell membrane, Shedding may also lead to a soluble peptide.

UniProt: Q9H5V8