Free Estriol Kit ELISA

N° du produit ABIN5696620

Le kit ELISA Free Estriol (ABIN5696620) Différentes espèces est un kit ELISA Colorimetric conçu pour quantifier Différentes espèces Free Estriol.

Aperçu rapide pour Free Estriol Kit ELISA (ABIN5696620)

Antigène: Free Estriol (Free Estriol (FE3))

Reactivité: Différentes espèces

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Détail du produit Free Estriol Kit ELISA

Seuil minimal de détection: 0.625 ng/mL

Fonction: F-E3 (Free Estriol) ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with F-E3 , no obvious cross reaction with other analogues.

Sensibilité: 0.375 ng/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Competitive ELISA, Coated with Antigen

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 50 µL standard or sample into each well. Immediately add 50 µL biotin-labeled antibody working solution into each well(The tip can't be reused after touching the liquid.), gently shake the plate for 1 min, seal closely and statically incubate for 45 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: Free Estriol (Free Estriol (FE3))

Autre désignation: F-E3 (Free Estriol)

Sujet: F-E3, Free Estriol