beta 2 Defensin Kit ELISA

N° du produit ABIN5706901

Kit ELISA Rat beta 2 Defensin, test Colorimetric pour la quantification de Rat beta 2 Defensin.

Aperçu rapide pour beta 2 Defensin Kit ELISA (ABIN5706901)

Antigène: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit beta 2 Defensin Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Analytical Method: Quantitative

Sensibilité: 0.061 ng/mL

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Volume d'échantillon: 100 µL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Autre désignation: Defensin Beta 2 (DEFb2)

Sujet: Alternative name: B-DF2, DEFB2, BD2, DEF-B2, HBD2, DEFB102, HBD-2, SAP1, DEFB4A, DEFB4, Skin-Antimicrobial Peptide 1