Myosin X Kit ELISA
Aperçu rapide pour Myosin X Kit ELISA (ABIN5706904)
Antigène
Tous les produits Myosin X (MYO10)Reactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.312 ng/mL
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Fonction
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MYO10 in human serum, plasma, tissue homogenates
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Analytical Method
- Quantitative
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Sensibilité
- 0.114 ng/mL
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual -
Volume d'échantillon
- 50 µL
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Durée du test
- 1 - 4.5 h
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Plaque
- Pre-coated
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Protocole
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1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately. -
Restrictions
- For Research Use only
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Stock
- 4 °C
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Date de péremption
- 12 months
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- Myosin X (MYO10)
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Sujet
- Alternative name: Unconventional Myosin-X, Unconventional Myosin-10
Antigène Tous les produits Myosin X (MYO10)
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