NMDAR2A Kit ELISA

N° du produit ABIN5706916

Kit ELISA Rat NMDAR2A, test Colorimetric pour la quantification de Rat NMDAR2A.

Aperçu rapide pour NMDAR2A Kit ELISA (ABIN5706916)

Antigène: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Détail du produit NMDAR2A Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Analytical Method: Quantitative

Sensibilité: 0.069 ng/mL

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Volume d'échantillon: 100 µL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Autre désignation: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A)

Sujet: Alternative name: NMDAR2A, NR2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity