Estrogen Receptor alpha Kit ELISA
Aperçu rapide pour Estrogen Receptor alpha Kit ELISA (ABIN6227021)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 62.5 pg/mL
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Fonction
- Pig Estrogen Receptor Alpha (ESR1) ELISA Kit
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Analytical Method
- Quantitative
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Sensibilité
- 37.5 pg/mL
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Ingrédients
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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Matériel non inclus
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Indications d'application
- Optimal dilutions/concentrations should be determined by the end user.
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Commentaires
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The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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Plaque
- Pre-coated
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Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of ER alpha were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of ER alpha were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<10%
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
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Date de péremption
- 6 months
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- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
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Autre désignation
- Estrogen Receptor Alpha
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Pathways
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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