Oxytocin Kit ELISA

N° du produit ABIN6244369

Le kit ELISA Oxytocin (ABIN6244369) Humain, Souris et Rat est un kit ELISA Colorimetric conçu pour quantifier Humain, Souris et Rat Oxytocin.

Aperçu rapide pour Oxytocin Kit ELISA (ABIN6244369)

Antigène: Oxytocin (OXT)

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Oxytocin Kit ELISA

Fonction: Human/Mouse/Rat Oxytocin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat Oxytocin

Sensibilité: 3.6 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samplesHuman: sample extraction / Mouse: sample extraction / Rat: sample extraction

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: Oxytocin (OXT)

Autre désignation: Oxytocin

Classe de substances: Hormone

Sujet:

Gene Symbols: OXT, OT

Background: Oxytocin is a nine amino acids peptide hormone and neuropeptide. The active oxytocin is cleaved from oxytocin- neurophysin I precursor. It regulates social bonding and sexual reproduction, playing a role in behaviors from maternal-infant bonding and milk release to empathy, generosity, and orgasm. Oxytocin is released during labor and with stimulation of the nipples from breastfeeding, and causes contraction of the smooth muscle of the uterus and of the mammary gland. This helps with birth, bonding with the baby, and milk production. Oxytocin is also used as a medication to facilitate childbirth.

Protein Name (Synonyms): Oxytocin-neurophysin 1 (OT-NPI) [Cleaved into: Oxytocin (Ocytoci

ID gène: 5020

Pathways: Myometrial Relaxation and Contraction, Feeding Behaviour