CCL1 Kit ELISA

N° du produit ABIN624994

Détail du produit CCL1 Kit ELISA

Antigène: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4-3000 pg/mL

Seuil minimal de détection: 4 pg/mL

Application: ELISA

Fonction: Human I-309 (TCA-3/CCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensibilité: 4 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour CCL1 Kit ELISA (ABIN624994)

Das, Sarrou, Podgrabinska, Cassella, Mungamuri, Feirt, Gordon, Nagi, Wang, Entenberg, Condeelis, Skobe: "Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses." dans: The Journal of experimental medicine, Vol. 210, Issue 8, pp. 1509-28, (2013) (PubMed).

Leow, Goh, Heng: "Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages." dans: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 61, Issue 4, pp. 391-8, (2012) (PubMed).

Celebi, Mantovani, Pineault: "Irradiated Mesenchymal Stem Cells improve the ex vivo expansion of Hematopoietic Progenitors by partly mimicking the bone marrow endosteal environment." dans: Journal of immunological methods, Vol. 370, Issue 1-2, pp. 93-103, (2011) (PubMed).

Détail du antigène

Antigène: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Autre désignation: I-309 (CCL1 Produits)

Synonymes: I-309 Kit ELISA, P500 Kit ELISA, SCYA1 Kit ELISA, SISe Kit ELISA, TCA3 Kit ELISA, BF534335 Kit ELISA, Scya1 Kit ELISA, Tca-3 Kit ELISA, CCLi6 Kit ELISA, chemokine Kit ELISA, C-C motif chemokine ligand 1 Kit ELISA, chemokine (C-C motif) ligand 1 Kit ELISA, CCL1 Kit ELISA, Ccl1 Kit ELISA

Sujet: Gene Names: CCL1 SCYA1
Protein names: C-C motif chemokine 1 (Small-inducible cytokine A1) (T lymphocyte-secreted protein I-309)

ID gène: 6346

UniProt: P22362