CCL3 Kit ELISA

N° du produit ABIN625046

Détail du produit CCL3 Kit ELISA

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6-3000 pg/mL

Seuil minimal de détection: 6 pg/mL

Application: ELISA

Fonction: Human MIP-1 alpha (CCL3) ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensibilité: 6 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

Collect plasma using heparin and/or EDTA as the anticoagulant. Citrate is not recommended.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour CCL3 Kit ELISA (ABIN625046)

Lu, Zheng, Fang, Huang, Ke, Wang, Zeng, Zheng, Yang: "Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays." dans: Journal of neuroimmunology, Vol. 293, pp. 39-44, (2016) (PubMed).

Wang, Su, Yang, Qiao, Fang, Yu, Yang, Wang, Yin, Chen, Hong: "The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery." dans: International journal of cancer, Vol. 138, Issue 11, pp. 2688-99, (2016) (PubMed).

Han, Li, Chen: "Genetic polymorphism of CCL2-2510 and susceptibility to enterovirus 71 encephalitis in a Chinese population." dans: Archives of virology, Vol. 159, Issue 9, pp. 2503-7, (2014) (PubMed).

Leow, Goh, Heng: "Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages." dans: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 61, Issue 4, pp. 391-8, (2012) (PubMed).

Weber: "[Metal ceramic boundary surfaces in the Ni-Cr-ceramic system from a recent viewpoint]." dans: Deutsche zahnärztliche Zeitschrift, Vol. 37, Issue 11, pp. 896-9, (1983) (PubMed).

Détail du antigène

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Autre désignation: MIP-1 alpha (CCL3 Produits)

Synonymes: MIP-1alpha Kit ELISA, G0S19-1 Kit ELISA, LD78ALPHA Kit ELISA, MIP-1-alpha Kit ELISA, MIP1A Kit ELISA, SCYA3 Kit ELISA, MIP-1a Kit ELISA, Scya3 Kit ELISA, AI323804 Kit ELISA, LD78alpha Kit ELISA, MIP1-(a) Kit ELISA, MIP1-alpha Kit ELISA, Mip1a Kit ELISA, CCL3L1 Kit ELISA, CCL3L3 Kit ELISA, chemokine (C-C motif) ligand 3 Kit ELISA, C-C motif chemokine ligand 3 Kit ELISA, CCL3 Kit ELISA, Ccl3 Kit ELISA

Sujet: Gene Names: CCL3 G0S19-1 MIP1A SCYA3
Protein names: C-C motif chemokine 3 (G0/G1 switch regulatory protein 19-1) (Macrophage inflammatory protein 1-alpha) (MIP-1-alpha) (PAT 464.1) (SIS-beta) (Small-inducible cytokine A3) (Tonsillar lymphocyte LD78 alpha protein) [Cleaved into: MIP-1-alpha(4-69) (LD78-alpha(4-69))]

ID gène: 6348

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy