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MMP1 Kit ELISA

MMP1 Reactivité: Humain Colorimetric Sandwich ELISA 8-18000 pg/mL Cell Lysate, Tissue Lysate
N° du produit ABIN625054
  • Antigène Voir toutes MMP1 Kits ELISA
    MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
    Reactivité
    • 9
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    8-18000 pg/mL
    Seuil minimal de détection
    8 pg/mL
    Application
    ELISA
    Fonction
    Human MMP-1 ELISA Kit for cell and tissue lysate samples.
    Type d'échantillon
    Tissue Lysate, Cell Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human MMP-1.
    Sensibilité
    8 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    • Cell lysate buffer
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes MMP1 Kits ELISA
    MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
    Autre désignation
    MMP-1 (MMP1 Produits)
    Synonymes
    CLG Kit ELISA, CLGN Kit ELISA, Mmp1a Kit ELISA, CG4859 Kit ELISA, Dm1-MMP Kit ELISA, Dmel\\CG4859 Kit ELISA, MMP-1 Kit ELISA, MMP1 Kit ELISA, Mmp 1 Kit ELISA, dMMP1 Kit ELISA, dm1-MMP Kit ELISA, dmmp1 Kit ELISA, l(2)k04809 Kit ELISA, mmp1 Kit ELISA, Mmp1 Kit ELISA, Mcol-A Kit ELISA, Mcola Kit ELISA, col4 Kit ELISA, mmp18 Kit ELISA, Clgn Kit ELISA, matrix metallopeptidase 1 Kit ELISA, Matrix metalloproteinase 1 Kit ELISA, matrix metalloproteinase 1 Kit ELISA, matrix metallopeptidase 1 (interstitial collagenase) Kit ELISA, matrix metallopeptidase 1a (interstitial collagenase) Kit ELISA, matrix metallopeptidase 1 S homeolog Kit ELISA, interstitial collagenase Kit ELISA, matrix metallopeptidase 8 L homeolog Kit ELISA, MMP1 Kit ELISA, Mmp1 Kit ELISA, RB11133 Kit ELISA, Mmp1a Kit ELISA, mmp1.S Kit ELISA, LOC100727966 Kit ELISA, mmp8.L Kit ELISA
    Sujet
    Gene Names: MMP1 CLG
    Protein names: Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Cleaved into: 22 kDa interstitial collagenase, 27 kDa interstitial collagenase]
    ID gène
    4312
    UniProt
    P03956
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