MMP13 Kit ELISA

N° du produit ABIN625055

Kit ELISA Humain MMP13, test Colorimetric pour la quantification de Humain MMP13 et a été mentionné dans 8+ publications.

Aperçu rapide pour MMP13 Kit ELISA (ABIN625055)

Antigène: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6-6000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Détail du produit MMP13 Kit ELISA

Seuil minimal de détection: 6 pg/mL

Fonction: Human MMP-13 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with any of the cytokines tested Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.

Sensibilité: 6 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour MMP13 Kit ELISA (ABIN625055)

Chijimatsu, Kunugiza, Taniyama, Nakamura, Tomita, Yoshikawa: "Expression and pathological effects of periostin in human osteoarthritis cartilage." dans: BMC musculoskeletal disorders, Vol. 16, pp. 215, (2015) (PubMed).

Soydinç, Sak, Evliyaoğlu, Evsen, Turgut, Ozler, Tay, Gül: "Maternal plasma prolidase, matrix metalloproteinases 1 and 13, and oxidative stress levels in pregnancies complicated by preterm premature rupture of the membranes and chorioamnionitis." dans: Journal of the Turkish German Gynecological Association, Vol. 13, Issue 3, pp. 172-7, (2014) (PubMed).

Zhang, Zhu, Chen, Zhang, Zhang, Jia, Wang, Liu, Xiao: "Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes." dans: International immunopharmacology, Vol. 21, Issue 2, pp. 301-8, (2014) (PubMed).

Balasubramanian, Pye, Willcox: "Effects of eye rubbing on the levels of protease, protease activity and cytokines in tears: relevance in keratoconus." dans: Clinical & experimental optometry : journal of the Australian Optometrical Association, Vol. 96, Issue 2, pp. 214-8, (2013) (PubMed).

Yuan, Zhao, Yuan, Che, Duan, Liu, Dong: "Association of endothelin-1 expression and cartilaginous endplate degeneration in humans." dans: PLoS ONE, Vol. 8, Issue 4, pp. e60062, (2013) (PubMed).

Rhim, Ahn, Kim, Kim, Lee, Kim, Kim, Park: "Stimulation of matrix metalloproteinases by tumor necrosis factor-? in human pulp cell cultures." dans: Journal of endodontics, Vol. 39, Issue 6, pp. 795-800, (2013) (PubMed).

Toegel, Wu, Otero, Goldring, Leelapornpisid, Chiari, Kolb, Unger, Windhager, Viernstein: "Caesalpinia sappan extract inhibits IL1?-mediated overexpression of matrix metalloproteinases in human chondrocytes." dans: Genes & nutrition, Vol. 7, Issue 2, pp. 307-18, (2012) (PubMed).

Jacob, Eisemon, Sheibani-Rad, Patel, Jacob, Choueka: "Matrix metalloproteinase levels as a marker for rotator cuff tears." dans: Orthopedics, Vol. 35, Issue 4, pp. e474-8, (2012) (PubMed).

Détail du antigène

Antigène: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Autre désignation: MMP-13

Sujet: Gene Names: MMP13
Protein names: Collagenase 3 (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13)

ID gène: 4322

UniProt: P45452