IL10RB Kit ELISA

N° du produit ABIN625310

Détail du produit IL10RB Kit ELISA

Antigène: IL10RB (Interleukin 10 Receptor, beta (IL10RB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 5-3000 pg/mL

Seuil minimal de détection: 5 pg/mL

Application: ELISA

Fonction: Human IL-10 R beta ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensibilité: 5 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL10RB (Interleukin 10 Receptor, beta (IL10RB))

Autre désignation: IL-10 R beta (IL10RB Produits)

Synonymes: IL10R2 Kit ELISA, IL10RB Kit ELISA, il10r2 Kit ELISA, CDW210B Kit ELISA, CRF2-4 Kit ELISA, CRFB4 Kit ELISA, D21S58 Kit ELISA, D21S66 Kit ELISA, IL-10R2 Kit ELISA, 6620401D04Rik Kit ELISA, AI528744 Kit ELISA, Crfb4 Kit ELISA, D16H21S58 Kit ELISA, D21S58h Kit ELISA, Il10r2 Kit ELISA, RGD1560373 Kit ELISA, interleukin 10 receptor subunit beta Kit ELISA, interleukin 10 receptor subunit beta S homeolog Kit ELISA, interleukin-10 receptor subunit beta Kit ELISA, interleukin 10 receptor, beta Kit ELISA, IL10RB Kit ELISA, il10rb.S Kit ELISA, LOC473966 Kit ELISA, LOC478404 Kit ELISA, il10rb Kit ELISA, Il10rb Kit ELISA

Sujet: Gene Names: IL10RB CRFB4 D21S58 D21S66
Protein names: Interleukin-10 receptor subunit beta (IL-10 receptor subunit beta) (IL-10R subunit beta) (IL-10RB) (Cytokine receptor class-II member 4) (Cytokine receptor family 2 member 4) (CRF2-4) (Interleukin-10 receptor subunit 2) (IL-10R subunit 2) (IL-10R2) (CD antigen CDw210b)

ID gène: 3588

UniProt: Q08334