CD56 Kit ELISA
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- Antigène Voir toutes CD56 (NCAM1) Kits ELISA
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.5 pg/mL-150 ng/mL
- Seuil minimal de détection
- 0.5 pg/mL
- Application
- ELISA
- Fonction
- Human NCAM-1 (CD56) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- Sensibilité
- 0.5 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples10-100 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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Low neural exosomal levels of cellular survival factors in Alzheimer's disease." dans: Annals of clinical and translational neurology, Vol. 2, Issue 7, pp. 769-73, (2015) (PubMed).
: "Promoter variant rs2301228 on the neural cell adhesion molecule 1 gene confers risk of schizophrenia in Han Chinese." dans: Schizophrenia research, Vol. 160, Issue 1-3, pp. 88-96, (2014) (PubMed).
: "
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Low neural exosomal levels of cellular survival factors in Alzheimer's disease." dans: Annals of clinical and translational neurology, Vol. 2, Issue 7, pp. 769-73, (2015) (PubMed).
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- Antigène Voir toutes CD56 (NCAM1) Kits ELISA
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
- Autre désignation
- N-CAM1 (NCAM1 Produits)
- Synonymes
- CD56 Kit ELISA, MSK39 Kit ELISA, NCAM Kit ELISA, fj56g09 Kit ELISA, ncam Kit ELISA, ncam1 Kit ELISA, wu:fj56g09 Kit ELISA, NCAM1 Kit ELISA, n-cam Kit ELISA, E-NCAM Kit ELISA, NCAM-1 Kit ELISA, Ncam Kit ELISA, Cd56 Kit ELISA, N-CAM Kit ELISA, N-CAM-1 Kit ELISA, NCAM-C Kit ELISA, NCAMC Kit ELISA, N-CAM-1-B Kit ELISA, NCAM-1-B Kit ELISA, ncam1-a Kit ELISA, ncam1-b Kit ELISA, neural cell adhesion molecule 1 Kit ELISA, neural cell adhesion molecule 1a Kit ELISA, neural cell adhesion molecule 1 S homeolog Kit ELISA, neural cell adhesion molecule 1 L homeolog Kit ELISA, NCAM1 Kit ELISA, ncam1a Kit ELISA, ncam1 Kit ELISA, LOC100085373 Kit ELISA, ncam1.S Kit ELISA, Ncam1 Kit ELISA, ncam1.L Kit ELISA
- Sujet
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Gene Names: NCAM1 NCAM
Protein names: Neural cell adhesion molecule 1 (N-CAM-1) (NCAM-1) (CD antigen CD56)
- ID gène
- 4684
- UniProt
- P13591
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