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IL17 Receptor B Kit ELISA

IL17RB Reactivité: Souris Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625406
  • Antigène Voir toutes IL17 Receptor B (IL17RB) Kits ELISA
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Reactivité
    • 6
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.2-50 ng/mL
    Seuil minimal de détection
    0.2 ng/mL
    Application
    ELISA
    Fonction
    Mouse IL-17 RB ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse Adiponectin (ACRP30), ANGPTL1, ANGPTL3, Artemin, CCL28 (MEC), CD36 (SR-B3), Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17B, IL-22, MIP-1beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGF beta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes IL17 Receptor B (IL17RB) Kits ELISA
    IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
    Autre désignation
    IL-17 RB (IL17RB Produits)
    Synonymes
    IL17RB Kit ELISA, CRL4 Kit ELISA, EVI27 Kit ELISA, IL17BR Kit ELISA, IL17RH1 Kit ELISA, Evi27 Kit ELISA, IL-17ER Kit ELISA, IL-17Rh1 Kit ELISA, Il17br Kit ELISA, interleukin 17 receptor B Kit ELISA, IL17RB Kit ELISA, Il17rb Kit ELISA
    ID gène
    50905
    UniProt
    Q9JIP3
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