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HTRA2 Kit ELISA

HTRA2 Reactivité: Humain Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625463
  • Antigène Voir toutes HTRA2 Kits ELISA
    HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
    Reactivité
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.2-50 ng/mL
    Seuil minimal de détection
    0.2 ng/mL
    Application
    ELISA
    Fonction
    Human HTRA2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detect human HtrA2. Other species not determined yet.
    Sensibilité
    200 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes HTRA2 Kits ELISA
    HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
    Autre désignation
    HTRA2 (HTRA2 Produits)
    Synonymes
    CG8464 Kit ELISA, DmHtrA2 Kit ELISA, Dmel\\CG8464 Kit ELISA, HtrA2/Omi Kit ELISA, Omi Kit ELISA, dOmi Kit ELISA, domi Kit ELISA, htrA2 Kit ELISA, omi Kit ELISA, HTRA2 Kit ELISA, PRSS25 Kit ELISA, OMI Kit ELISA, PARK13 Kit ELISA, AI481710 Kit ELISA, Prss25 Kit ELISA, mnd2 Kit ELISA, HTRA2-related serine protease Kit ELISA, HtrA serine peptidase 2 Kit ELISA, HtrA2 Kit ELISA, HTRA2 Kit ELISA, htra2 Kit ELISA, Htra2 Kit ELISA
    Sujet
    Gene Names: HTRA2 OMI PRSS25
    Protein names: Serine protease HTRA2, mitochondrial (EC 3.4.21.108) (High temperature requirement protein A2) (HtrA2) (Omi stress-regulated endoprotease) (Serine protease 25) (Serine proteinase OMI)
    ID gène
    27429
    UniProt
    O43464
    Pathways
    Positive Regulation of Endopeptidase Activity
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