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PRSS22 Kit IQ-ELISA

PRSS22 Reactivité: Souris qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN6385868
  • Antigène Voir toutes PRSS22 IQ-ELISA Kits
    PRSS22 (Protease, serine, 22 (PRSS22))
    Reactivité
    • 1
    • 1
    Souris
    Méthode de détection
    qPCR
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse Immunoquantitative (PCR-Based) Tryptase epsilon ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Semi-Quantitative
    Sensibilité
    6 pg/mL
    Attributs du produit
    IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
    Ingrédients
    • Tryptase epsilon Microplate (Item A): 96 well PCR plate coated with anti-Mouse Tryptase epsilon
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Mouse Tryptase epsilon
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for Tryptase epsilon (Item F): 2 vials of a 4x concentrated solution of anti-Mouse Tryptase epsilon affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    Matériel non inclus
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
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  • Indications d'application
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    Commentaires

    The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

    Volume d'échantillon
    25 µL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes PRSS22 IQ-ELISA Kits
    PRSS22 (Protease, serine, 22 (PRSS22))
    Autre désignation
    Tryptase epsilon (PRSS22 Produits)
    Synonymes
    BSSP-4 Kit IQ-ELISA, hBSSP-4 Kit IQ-ELISA, 4733401N09Rik Kit IQ-ELISA, SP001LA Kit IQ-ELISA, protease, serine, 22 Kit IQ-ELISA, protease, serine 22 Kit IQ-ELISA, Prss22 Kit IQ-ELISA, PRSS22 Kit IQ-ELISA
    ID gène
    70835
    UniProt
    Q9ER10
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