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Nociceptin Kit ELISA

PNOC Reactivité: Humain, Rat, Souris Colorimetric Competition ELISA 0.1 ng/mL - 1000 ng/mL Cell Culture Supernatant, Serum
N° du produit ABIN6386187
  • Antigène Voir toutes Nociceptin (PNOC) Kits ELISA
    Nociceptin (PNOC)
    Reactivité
    • 5
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    0.1 ng/mL - 1000 ng/mL
    Seuil minimal de détection
    0.1 ng/mL
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Nociceptin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This EIA kit is designed to detect human, mouse, and rat Nociceptin
    Sensibilité
    0.4 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Préparation des réactifs

    Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Date de péremption
    6 months
  • Antigène Voir toutes Nociceptin (PNOC) Kits ELISA
    Nociceptin (PNOC)
    Autre désignation
    Nociceptin (PNOC Produits)
    Synonymes
    PPNOC Kit ELISA, N/OFQ Kit ELISA, N23K Kit ELISA, Npnc1 Kit ELISA, OFQ/N Kit ELISA, pnoc Kit ELISA, zgc:136385 Kit ELISA, OFQ Kit ELISA, prepronociceptin Kit ELISA, prepronociceptin b Kit ELISA, PNOC Kit ELISA, Pnoc Kit ELISA, pnocb Kit ELISA
    ID gène
    5368
    UniProt
    Q13519
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