BMP2 Kit ELISA

N° du produit ABIN6719673

Détail du produit BMP2 Kit ELISA

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Bone, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: BDA2, BMP-2, BMP-2A, Bmp2, BMP2_HUMAN, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A

Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Gene Name: BMP2

Production: Natural and recombinant mouse BMP-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard:CHO , Immunogen sequence: Q283-R398

Endotoxin level: Low endotoxin

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse bone tissue, cell culture supernates or serum to each empty well. It is recommended that each mouse BMP-2 standard solution and each sample be measured in duplicate.

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Autre désignation: BMP-2 (BMP2 Produits)

Synonymes: BDA2 Kit ELISA, BMP2A Kit ELISA, AI467020 Kit ELISA, Bmp2a Kit ELISA, BMP-2 Kit ELISA, xBMP-2 Kit ELISA, xbmp2 Kit ELISA, BMP2 Kit ELISA, bmp2a Kit ELISA, bmp2 Kit ELISA, wu:fc59d09 Kit ELISA, bone morphogenetic protein 2 Kit ELISA, bone morphogenetic protein 2 L homeolog Kit ELISA, Bone morphogenetic protein 2 Kit ELISA, bone morphogenetic protein 2a Kit ELISA, BMP2 Kit ELISA, Bmp2 Kit ELISA, bmp2.L Kit ELISA, bmp2 Kit ELISA, bmp2a Kit ELISA

ID gène: 12156

NCBI Accession: NP_031579

UniProt: P21274

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation