SPARCL1 Kit ELISA

N° du produit ABIN6719689

N° du produit (Fournisseur): koa0783

Kit ELISA SPARCL1 Humain, Colorimetric test pour la quantification de Humain SPARCL1.

Aperçu rapide pour SPARCL1 Kit ELISA (ABIN6719689)

Antigène: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit SPARCL1 Kit ELISA

Seuil minimal de détection: 46.9 pg/mL

N° du produit (Fournisseur): koa0783

Fournisseur: Rockland

Fonction: Human SPARCL1 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: I17-F664

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL human SPARCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. We recommend that each human SPARCL1 standard solution and each sample is measured in duplicate. 2. Seal the plate with the cover and incubate at 37 °C for 90 min.

Précision du teste: Intra-Assay Precision (CV%): 5.5%, 5.5%, 6.7%
Inter-Assay Precision (CV%): 6.1%, 6.7%, 7.1%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Autre désignation: SPARCL1

Sujet:

Background: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Gene Full Name: SPARC like 1

ID gène: 8404

NCBI Accession: NP_001121782

UniProt: Q14515