HAVCR1 Kit ELISA

N° du produit ABIN6719804

Détail du produit HAVCR1 Kit ELISA

Antigène: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate, Urine

Analytical Method: Quantitative

Attributs du produit:

Synonyms: HAVCR, HAVcr-1, Havcr1, HAVR1_HUMAN, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM-1, T cell immunoglobin domain and mucin domain protein 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM-1, TIM1, TIMD-1, TIMD1

Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.

Gene Name: HAVCR1

Production: Natural and recombinant rat KIM1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S18-V238

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each rat KIM1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Autre désignation: KIM1 (HAVCR1 Produits)

Synonymes: HAVCR Kit ELISA, HAVCR-1 Kit ELISA, KIM-1 Kit ELISA, KIM1 Kit ELISA, TIM Kit ELISA, TIM-1 Kit ELISA, TIM1 Kit ELISA, TIMD-1 Kit ELISA, TIMD1 Kit ELISA, Kim1 Kit ELISA, HAVCR1 Kit ELISA, LOC100226241 Kit ELISA, AI503787 Kit ELISA, Tim1 Kit ELISA, Timd1 Kit ELISA, hepatitis A virus cellular receptor 1 Kit ELISA, hepatitis A virus cellular receptor 1 homolog Kit ELISA, HAVCR1 Kit ELISA, Havcr1 Kit ELISA, LOC100226241 Kit ELISA

Classe de substances: Virus

ID gène: 286934

NCBI Accession: NP_775172

UniProt: O54947