ICAM1 Kit ELISA

N° du produit ABIN6719826

Détail du produit ICAM1 Kit ELISA

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Lysates

Analytical Method: Quantitative

Attributs du produit:

Synonyms: BB2, CD 54, CD54, CD54 antigen, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM 1, Intercellular adhesion molecule 1, Major group rhinovirus receptor, MALA2, MGC6195, MyD10, P3.58, Surface antigen of activated B cells

Background: Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8 kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.

Gene Name: ICAM1

Production: Natural and recombinant mouse sICAM-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q28-N485

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ICAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin, EDTA) or tissue lysates to each empty well. It is recommended that each mouse ICAM-1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: ICAM-1 (ICAM1 Produits)

Synonymes: BB2 Kit ELISA, CD54 Kit ELISA, P3.58 Kit ELISA, Icam-1 Kit ELISA, Ly-47 Kit ELISA, MALA-2 Kit ELISA, ICAM Kit ELISA, ICAM-1 Kit ELISA, ICAM1 Kit ELISA, intercellular adhesion molecule 1 Kit ELISA, intercellular adhesion molecule-1 Kit ELISA, ICAM1 Kit ELISA, Icam1 Kit ELISA, ICAM-1 Kit ELISA

Classe de substances: Viral Protein

ID gène: 15894

NCBI Accession: NP_034623

UniProt: P13597

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling