CD47 Kit ELISA

N° du produit ABIN6719833

Détail du produit CD47 Kit ELISA

Antigène: CD47

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Antigen identified by monoclonal antibody 1D8, Antigenic surface determinant protein OA3, CD 47, CD47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 Molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen

Background: CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.

Gene Name: CD47

Production: Natural and recombinant human CD47. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q19-P139

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD47 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human CD47 standard solution and each sample be measured in duplicate.

ELISA Dilution: 125pg/mL-8000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CD47

Autre désignation: CD47 (CD47 Produits)

Synonymes: IAP Kit ELISA, MER6 Kit ELISA, OA3 Kit ELISA, 9130415E20Rik Kit ELISA, AA407862 Kit ELISA, AI848868 Kit ELISA, AW108519 Kit ELISA, B430305P08Rik Kit ELISA, Itgp Kit ELISA, CD47/IAP Kit ELISA, alkaline phosphatase, intestinal Kit ELISA, CD47 molecule Kit ELISA, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) Kit ELISA, Cd47 molecule Kit ELISA, ALPI Kit ELISA, CD47 Kit ELISA, Cd47 Kit ELISA

ID gène: 961

NCBI Accession: NP_001768

UniProt: Q08722