CD56 Kit ELISA
Aperçu rapide pour CD56 Kit ELISA (ABIN6719883)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 156 pg/mL
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N° du produit (Fournisseur)
- koa0409
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Fournisseur
- Rockland
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Fonction
- Human CD56 - NCAM-1 Sandwich ELISA Kit for Quantitative Detection
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Marque
- AccuSignal™
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Analytical Method
- Quantitative
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Specificité
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Production: Natural and recombinant human CD56. There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Indications d'application
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ELISA_Dilution: 156 pg/mL-10000 pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CD56 standard solution and each sample is measured in duplicate.
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Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: L20-G718
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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: "NCAM1 and GDF15 are biomarkers of Charcot-Marie-Tooth disease in patients and mice." dans: Brain : a journal of neurology, Vol. 145, Issue 11, pp. 3999-4015, (2022) (PubMed).
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: "NCAM1 and GDF15 are biomarkers of Charcot-Marie-Tooth disease in patients and mice." dans: Brain : a journal of neurology, Vol. 145, Issue 11, pp. 3999-4015, (2022) (PubMed).
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- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
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Autre désignation
- CD56/NCAM-1
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Sujet
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Synonyms: CD56, N-CAM-1, NCAM-1, NCAM1, NCAM1_HUMAN, Neural cell adhesion molecule 1
Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
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ID gène
- 4684
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NCBI Accession
- NP_000606
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UniProt
- P13591
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