Sialoadhesin/CD169 Kit ELISA

N° du produit ABIN6719887

N° du produit (Fournisseur): koa0778

Kit ELISA Humain Sialoadhesin/CD169, test Colorimetric pour la quantification de Humain Sialoadhesin/CD169.

Aperçu rapide pour Sialoadhesin/CD169 Kit ELISA (ABIN6719887)

Antigène: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Détail du produit Sialoadhesin/CD169 Kit ELISA

Seuil minimal de détection: 156 pg/mL

N° du produit (Fournisseur): koa0778

Fournisseur: Rockland

Fonction: Human CD169 - SIGLEC-1 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CD169. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD169 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD169 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: S20-Q1641

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Autre désignation: CD169/SIGLEC-1

Sujet:

Synonyms: CD169, CD169 antigen, dJ1009E24.1, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, Sialic acid binding Ig like lectin 1, Sialic acid binding immunoglobulin like lectin 1, Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec 1, Siglec-1, Siglec1, SN, SN_HUMAN

Background: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

ID gène: 6614

NCBI Accession: NP_075556

UniProt: Q9BZZ2