CRLF2 Kit ELISA

N° du produit ABIN6719890

Détail du produit CRLF2 Kit ELISA

Antigène: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CRL 2, CRL2, CRLF 2, Crlf2, CRLF2_HUMAN, CRLF2Y, Cytokine receptor CRL2 precusor, Cytokine receptor like 2, Cytokine receptor like factor 2, Cytokine receptor like factor 2 precursor, Cytokine receptor-like 2, Cytokine receptor-like factor 2, IL XR, IL-XR, ILXR, Thymic stromal derived lymphopoietin receptor, Thymic stromal lymphopoietin protein receptor, Thymic stromal lymphopoietin receptor, TSLP receptor, TSLPR

Background: Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin(TSLP). Together with the interleukin 7 receptor(IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

Gene Name: CRLF2

Production: Natural and recombinant human CRLF2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G25-K231

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CRLF2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CRLF2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Autre désignation: CRLF2 - TSLP R (CRLF2 Produits)

Synonymes: CRLF2 Kit ELISA, CRL2 Kit ELISA, CRLF2Y Kit ELISA, TSLPR Kit ELISA, CRLM2 Kit ELISA, Ly114 Kit ELISA, Tpte2 Kit ELISA, Tslpr Kit ELISA, Crl2 Kit ELISA, cytokine receptor like factor 2 Kit ELISA, cytokine receptor-like factor 2 Kit ELISA, CRLF2 Kit ELISA, Crlf2 Kit ELISA

ID gène: 64109

NCBI Accession: NP_001012288

UniProt: Q9HC73