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DKK3 Kit ELISA

Reactivité: Humain Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
N° du produit ABIN6719891
  • Antigène Voir toutes DKK3 Kits ELISA
    DKK3
    Reactivité
    • 4
    • 3
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156 pg/mL - 10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: AW061014, C87148, Dickkopf (Xenopus laevis) homolog 3, Dickkopf 3, Dickkopf homolog 3 (Xenopus laevis), Dickkopf related protein 3, HDkk 3, Regulated in glioma, REIC, RIG, RIG like 5 6, RIG like 7 1

    Background: Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

    Gene Name: DKK3

    Production: Natural and recombinant human DKK-3. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: A22-I350

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  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human DKK-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DKK-3 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 156pg/mL-10,000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes DKK3 Kits ELISA
    DKK3
    Autre désignation
    DKK-3 (DKK3 Produits)
    Synonymes
    REIC Kit ELISA, RIG Kit ELISA, reic Kit ELISA, dkk-3 Kit ELISA, AW061014 Kit ELISA, C87148 Kit ELISA, Reic Kit ELISA, p29 Kit ELISA, dickkopf WNT signaling pathway inhibitor 3 Kit ELISA, dickkopf WNT signaling pathway inhibitor 3 S homeolog Kit ELISA, DKK3 Kit ELISA, dkk3 Kit ELISA, dkk3.S Kit ELISA, Dkk3 Kit ELISA
    ID gène
    27122
    NCBI Accession
    NP_001018067
    UniProt
    Q9UBP4
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