GAL4 Kit ELISA

N° du produit ABIN6719895

Détail du produit GAL4 Kit ELISA

Antigène: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Antigen NY-CO-27, Gal-4, Galectin-4, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4, LEG4_HUMAN, LGALS4

Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

Gene Name: LGALS4

Production: Natural and recombinant human Galectin-4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A2-I323

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Galectin-4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Autre désignation: Galectin-4 (LGALS4 Produits)

Synonymes: LGALS4 Kit ELISA, gal4 Kit ELISA, l36lbp Kit ELISA, MGC86186 Kit ELISA, galectin-4 Kit ELISA, xgalectin-VIa Kit ELISA, gal-4 Kit ELISA, L36LBP Kit ELISA, L-36 Kit ELISA, L36LBI Kit ELISA, GAL4 Kit ELISA, galectin 4 Kit ELISA, lectin, galactoside-binding, soluble, 4 L homeolog Kit ELISA, Galectin-4 Kit ELISA, lectin, galactose binding, soluble 4 Kit ELISA, LGALS4 Kit ELISA, lgals4.L Kit ELISA, leg4 Kit ELISA, Lgals4 Kit ELISA

ID gène: 3960

NCBI Accession: NP_006140

UniProt: P56470