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LOXL2 Kit ELISA

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain LOXL2.
Rockland
N° du produit ABIN6719903
N° du produit (Fournisseur): koa0838

Aperçu rapide pour LOXL2 Kit ELISA (ABIN6719903)

Antigène

Voir toutes LOXL2 Kits ELISA
LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Reactivité

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Humain

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

156 pg/mL - 10000 pg/mL

Application

ELISA

Type d'échantillon

Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
  • Seuil minimal de détection

    156 pg/mL

    N° du produit (Fournisseur)

    koa0838

    Fournisseur

    Rockland

    Fonction

    Human LOXL2 Sandwich ELISA Kit for Quantitative Detection

    Marque

    AccuSignal™

    Analytical Method

    Quantitative

    Specificité

    Production: Natural and recombinant human LOXL2. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité

    < 10 pg/mL

    Ingrédients

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Indications d'application

    ELISA_Dilution: 156pg/mL-10,000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human LOXL2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human LOXL2 standard solution and each sample be measured in duplicate.

    Commentaires

    Standard: Expression system for standard: NSO, Immunogen sequence: Q26-Q774

    Volume d'échantillon

    100 μL

    Plaque

    Pre-coated

    Restrictions

    For Research Use only
  • Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Date de péremption

    12 months
  • Antigène Voir toutes LOXL2 Kits ELISA

    LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

    Autre désignation

    LOXL2

    Sujet

    Synonyms: LOR 2, LOR2, LOX L2, LOXL 2, LOXL2, LOXL2_HUMAN, Lysyl oxidase homolog 2, Lysyl oxidase like 2, Lysyl oxidase like protein 2, Lysyl oxidase related 2, Lysyl oxidase related protein 2, Lysyl oxidase related protein WS9 14, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, WS9 14

    Background: Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.

    ID gène

    4017

    NCBI Accession

    NP_002309

    UniProt

    Q9Y4K0

    Pathways

    Chromatin Binding
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