SHBG Kit ELISA

N° du produit ABIN6719904

N° du produit (Fournisseur): koa0728

Le kit ELISA SHBG (ABIN6719904) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain SHBG.

Aperçu rapide pour SHBG Kit ELISA (ABIN6719904)

Antigène: SHBG (Sex Hormone Binding Globulin (SHBG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.063 nmol/L - 4 nmol/L

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit SHBG Kit ELISA

Seuil minimal de détection: 0.063 nmol/L

N° du produit (Fournisseur): koa0728

Fournisseur: Rockland

Fonction: Human SHBG Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: L30-H402

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 0.01 nmol/L

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2nmol/L, 1nmol/L, 0.5nmol/L, 0.25nmol/L, 0.125nmol/L, 0.062nmol/L, 0.031nmol/L human SHBG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human SHBG standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 3.0%, 2.5%, 2.9%
Inter-Assay Precision (CV%): 6.8%, 3.4%, 2.8%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: SHBG (Sex Hormone Binding Globulin (SHBG))

Autre désignation: SHBG

Sujet:

Background: Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12→p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.

Gene Full Name: Sex hormone-binding globulin

ID gène: 6462

NCBI Accession: NP_001031

UniProt: P04278

Pathways: Hormone Transport