BMP5 Kit ELISA

N° du produit ABIN6719905

Détail du produit BMP5 Kit ELISA

Antigène: BMP5 (Bone Morphogenetic Protein 5 (BMP5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Bone, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: BMP-5, Bmp5, BMP5_HUMAN, Bone morphogenetic protein 5

Background: Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.

Gene Name: BMP5

Production: Natural and recombinant human BMP-5. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A317-H454

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human BMP-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or bone tissue to each empty well. It is recommended that each human BMP-5 standard solution and each sample be measured in duplicate.

ELISA Dilution: 156pg/mL-10000g/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: BMP5 (Bone Morphogenetic Protein 5 (BMP5))

Autre désignation: BMP-5 (BMP5 Produits)

Synonymes: bmp5l Kit ELISA, hm:zehl0669 Kit ELISA, zehl0669 Kit ELISA, zgc:64230 Kit ELISA, BMP5 Kit ELISA, AU023399 Kit ELISA, se Kit ELISA, bone morphogenetic protein 5 Kit ELISA, bmp5 Kit ELISA, BMP5 Kit ELISA, LOC100539495 Kit ELISA, Bmp5 Kit ELISA

ID gène: 653

NCBI Accession: NP_066551

UniProt: P22003

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process