TGFB2 Kit ELISA

N° du produit ABIN6719917

N° du produit (Fournisseur): koa0610

Kit ELISA TGFB2 Rat, Colorimetric test pour la quantification de Rat TGFB2.

Aperçu rapide pour TGFB2 Kit ELISA (ABIN6719917)

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Détail du produit TGFB2 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0610

Fournisseur: Rockland

Fonction: Rat TGF beta 2 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: A303-S414

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each rat TGF-beta 2 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.6%, 7.5%, 4.6%
Inter-Assay Precision (CV%): 4.7%, 8.1%, 5%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Autre désignation: TGF-beta 2

Sujet:

Background: Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Gene Full Name: transforming growth factor, beta 2

ID gène: 81809

NCBI Accession: NP_112393

UniProt: Q07257

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus