CD166 Kit ELISA

N° du produit ABIN6719924

Détail du produit CD166 Kit ELISA

Antigène: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Activated leukocyte cell adhesion molecule, ALCAM, ALCAM protein, CD 166, CD166, CD166 antigen, CD166_HUMAN, FLJ3851, FLJ38514, MEMD, MGC71733

Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166(cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

Gene Name: ALCAM

Production: Natural and recombinant human ALCAM. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: W28-A526

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ALCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. We recommend that each human ALCAM standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Autre désignation: ALCAM (ALCAM Produits)

Synonymes: CD166 Kit ELISA, MEMD Kit ELISA, ALCAM Kit ELISA, BEN Kit ELISA, DM-GRASP Kit ELISA, JC7 Kit ELISA, SC1 Kit ELISA, AI853494 Kit ELISA, MuSC Kit ELISA, alcam Kit ELISA, cd166 Kit ELISA, memd Kit ELISA, fb53h09 Kit ELISA, fb80a10 Kit ELISA, nlcam Kit ELISA, si:ch211-190h2.4 Kit ELISA, wu:fb53h09 Kit ELISA, wu:fb80a10 Kit ELISA, wu:fc14b01 Kit ELISA, activated leukocyte cell adhesion molecule Kit ELISA, activated leukocyte cell adhesion molecule a Kit ELISA, activated leukocyte cell adhesion molecule L homeolog Kit ELISA, CD166 antigen homolog Kit ELISA, activated leukocyte cell adhesion molecule b Kit ELISA, ALCAM Kit ELISA, Alcam Kit ELISA, alcama Kit ELISA, alcam.L Kit ELISA, alcam Kit ELISA, cd166 Kit ELISA, alcamb Kit ELISA

ID gène: 214

NCBI Accession: NP_001230209

UniProt: Q13740