CEA Kit ELISA
Aperçu rapide pour CEA Kit ELISA (ABIN6719926)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 312 pg/mL
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N° du produit (Fournisseur)
- koa0544
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Fournisseur
- Rockland
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Fonction
- Human CEA Sandwich ELISA Kit for Quantitative Detection
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Marque
- AccuSignal™
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Analytical Method
- Quantitative
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Specificité
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Production: Natural and recombinant human CEA. There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human CEA standard solution and each sample be measured in duplicate.
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Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: K35-A685
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- CEA (Carcinoembryonic Antigen Gene Family (CEA))
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Autre désignation
- CEA
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Sujet
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Synonyms: Carcinoembryonic antigen , Carcinoembryonic antigen related cell adhesion molecule 5, CD66e, CD66e antigen, CEA, CEACAM 5, CEACAM5, DKFZp781M2392, Meconium antigen 100
Background: Carcinoembryonic antigen(CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60 % carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon. Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
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ID gène
- 1048
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NCBI Accession
- NP_001278413
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UniProt
- P06731
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