CEA Kit ELISA

N° du produit ABIN6719926

N° du produit (Fournisseur): koa0544

Kit ELISA CEA Humain, Colorimetric test pour la quantification de Humain CEA.

Aperçu rapide pour CEA Kit ELISA (ABIN6719926)

Antigène: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit CEA Kit ELISA

Seuil minimal de détection: 312 pg/mL

N° du produit (Fournisseur): koa0544

Fournisseur: Rockland

Fonction: Human CEA Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: K35-A685

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: polyclonal antibody from rabbit, polyclonal antibody from rabbit

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human CEA standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4%, 7.5%, 4%
Inter-Assay Precision (CV%): 5.4%, 8.3%, 4%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Autre désignation: CEA

Sujet:

Background: Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60 % carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Gene Full Name: CEA cell adhesion molecule 5

ID gène: 1048

NCBI Accession: NP_001278413

UniProt: P06731